3ZZ Oprm1 Probe From ACD for BaseScope-ISH in Mouse Samples

December 07, 2023

BlueskyReddit
State University of New York at Buffalo
Physiology and Biophysics
Postdoctoral Researcher

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Company:

Advanced Cell Diagnostics

Product Name:

BaseScope probe: BA-Mm-Oprm1-3zz-st-C2

Catalog Number:

1048301-C2

We used the Oprm1 3ZZ probe to target Oprm1 mRNA (GenBank accession NM_001039652.2; nucleotides 845–976) in mouse fresh frozen brain sections.

Experimental Design and Results Summary

Application

RNA-ISH

Starting Material

Mouse brain fresh frozen tissue

Tips

Keep in mind that dilution should be 1:50

Results Summary

This probe is able to detect Oprm1 mRNA in mouse brain samples (we tested in medial prefrontal cortex, hippocampus, caudate putamen, nucleus accumbens, and the ventral tegmental area. Note that this probe was used in combination with BaseScope Duplex Reagent Kit (chromogenic assay). Validated on knockout mice. Image: Oprm1 individual mRNA puncta surrounded by Aldh1 mRNA punctum (blue). Brain tissue was additionally stained with 50% Gill’s hematoxylin 1.

DOI or PMID #

37408246

Image Gallery

Summary

The Good

The utilization of a custom-designed "3ZZ" probe targeting Oprm1 mRNA ensures specificity.

The Bad

Low quantity, and expensive.

The Bottom Line

The BaseScope Oprm1 probe delivered a positive and reliable experience, characterized by clear and specific signals in the detection of Oprm1 mRNA. The probe demonstrated a high specificity for the target gene, ensuring confidence in the obtained results. While the effectiveness of the probe is commendable, it's essential to note that the technique comes at a premium cost.

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