ThermoFisher Scientific
Vimentin Monoclonal Antibody
MA5-11883
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We study multiple pathological processes in ocular diseases. The specific goal of this experiment was to evaluate vimentin protein expression in cell lysate samples . This antibody worked for western blotting as a clear single band for VIM was observed.
Western Blot
Mouse fibroblast lysate
1:1000 at 4 degrees Celsius for over night
5% normal donkey serum
Anti-mouse secondary antibody at 1:10000 for 1hr at room temp
N/A
ECL reagent
A single, very clean band from mouse fibroblast cell lysate samples was observed. This antibody had high specificity and is easy to optimise. I would definitely buy again. Don't need to use too much as it has high sensitivity.
Very robust signal
none
High specificity. I would recommend it for detecting Vimentin.