Decent BioLegend CCR6 staining on mouse lymphocytes

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD196 (CCR6) Antibody

Catalog Number:

129804

Clone Number:

29-2L17

Decent staining of CCR6-positive populations in mouse MC38 tumor-infiltrating lymphocytes. It is a useful antibody to detect TH17-like cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor MC38 cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

Single cell suspension of mouse lymphocytes from MC38 subcutaneous tumors stained with this antibody shows a decent shift separation between positive and negative populations. This is more a shift than a clear cute separate two populations, but this is not unusual with this marker.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Decent and specific staining

The Bad

The shift is decent but you may need titration to get the best staining intensity

The Bottom Line

Decent staining of CCR6 in mouse MC38 tumor-infiltrating lymphocytes. Useful to detect TH17-like cells

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