Nice Anti-Vimentin Antibody

May 25, 2022

Nanomedicine
Houston Methodist Research Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-Vimentin antibody

Catalog Number:

ab92547

Clone Number:

EPR3776

I used this antibody to check expression of vimentin in A549 lung cancer cells. I did western blot and immunofluorescence staining. This antibody works great.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A549 Human Lung Cancer Cell Line

Primary Incubation

1:5000

Blocking Agent

5% BSA

Secondary Incubation

1:5000

Tertiary Incubation

N/A

Detection

ChemiDoc Imaging Systems

Results Summary

The signal is good and the bands are very strong.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable

The Bad

Expensive

The Bottom Line

This is a nice antibody for vimentin. It can be used for wester blot, immunofluorescence staining, highly recommended.

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