Excellent hTNFa Antibody from BioLegend

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

APC anti-human TNF-α Antibody

Catalog Number:

502912

Clone Number:

N/A

We were phenotyping some human T cells isolated from PBMC-humanized mice, so we used hTNFa to assess cytokine secretion following in vitro stimulation. The antibody stains hTNFa+ cells very clearly, allowing us to compare the proportion of cytokine-secreting cells in different experimental conditions.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleen from PBMC-humanized mice

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer

Results Summary

The antibody stains hTNFa+ cells very clearly, allowing us to compare the proportion of cytokine-secreting cells in different experimental conditions.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining hTNFa.

The Bad

None to mention.

The Bottom Line

The product is great for assessing T cell cytokine secretion.

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