Antibody Brightly Stains hCD14

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-human CD14 Antibody

Catalog Number:

367124

Clone Number:

N/A

We wanted to phenotype human macrophages in an in vitro culture, so we compared hCD14 expression on human macrophages compared to monocytes. This antibody works great for staining hCD14.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

In vitro differentiated human macrophages

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Attune NxT cytometer

Results Summary

The antibody stained hCD14 on the differentiated macrophages very clearly, with much higher expression on the macrophages than on monocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining hCD14.

The Bad

None to mention.

The Bottom Line

The antibody very clearly stains hCD14, which is great for identifying human monocytes or macrophages.

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