Excellent Staining with hCD3 Antibody

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-human CD3 Antibody

Catalog Number:

317334

Clone Number:

N/A

We were phenotyping some human PBMCs, so we used hCD3 to pull out human T cells from other human immune cell populations. The antibody stains hCD3 very clearly, allowing identification and analysis of human T cells from the PBMC mixture.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer

Results Summary

The antibody stains hCD3 very clearly, allowing identification and analysis of human T cells from the PBMC mixture.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining hCD3.

The Bad

None to mention.

The Bottom Line

The product is great for identifying human T cells.

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