Time Consuming Kit, Low Sample Purity

Columbia University Medical Center
Microbiology and Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

StemCell Technologies

Product Name:

EasySep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog Number:

19159

We got a sample of this kit to test using it for enrichment of memory T cells prior to FACS for T cell stimulation experiments. We were able to get an enrichment of memory T cells, but the sample purity wasn't as high as we would have liked.

Experimental Design and Results Summary

Application

Cytokine Production Experiments

Starting Material

N/A

Tips

Time consuming.

Results Summary

We were able to get an enrichment of memory T cells, but the sample purity wasn't as high as we would have liked.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Clear instructions.

The Bad

Long incubation steps.

The Bottom Line

In total, there are 25 minutes worth of incubation steps when using this kit. I am not sure it is worth using. Perhaps a T cell enrichment before sorting is sufficient.

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