CD80 Antibody Works Very Well for Flow Cytometry

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-human CD80 Antibody

Catalog Number:

305238

Clone Number:

N/A

We wanted to phenotype human macrophages in an in vitro culture, so we compared hCD16 expression on human macrophages compared to monocytes. This antibody is great for checking the level of co-stimulation on human immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

In vitro differentiated human macrophages

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer

Results Summary

The antibody stains hCD80 on the differentiated macrophages very well. The receptor is upregulated on macrophages compared to monocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining hCD80.

The Bad

None to mention.

The Bottom Line

The antibody is great for checking the level of co-stimulation on human immune cells.

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