Kit Works with EasySep Magnet

May 26, 2022

BlueskyReddit
Columbia University Medical Center
Microbiology and Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

StemCell Technologies

Product Name:

EasySep™ Human T Cell Isolation Kit

Catalog Number:

17951

We use these T cell enrichment kits to enrich our samples before FACS to reduce our sorting times. The enriched samples are used for stimulation experiments or sequencing.

Experimental Design and Results Summary

Application

Whole Genome Bisulfite Sequencing

Starting Material

N/A

Tips

If using magnet other than EasySep, takes longer than 8 minutes.

Results Summary

I usually use the EasyEights magnet because I have several samples to sort, but I was surprised to see that if I used the EasyEights magnet, the total enrichment time would take at least 15 minutes. The advertisement was misleading.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Quick 8 minute enrichment if using EasySep magnet.

The Bad

Enrichment takes longer if using other magnets.

The Bottom Line

The kit works quickly and saves a lot of time only if you are using the EasySep magnet. If you are processing several samples at a time and need to use the EasyEights magnet, you could probably use a cheaper kit instead.

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