Assay Kit More Time Consuming Than Expected

May 26, 2022

BlueskyReddit
Columbia University Medical Center
Microbiology and Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

StemCell Technologies

Product Name:

EasySep™ Human T Cell Enrichment Kit

Catalog Number:

19051

We use these negative enrichment kits to enrich for T cells before FACS to reduce sorting time. The sorted cells are used for stimulation experiments or sequencing.

Experimental Design and Results Summary

Application

Whole Genome Bisulfite Sequencing

Starting Material

N/A

Tips

Many long incubation steps

Results Summary

The kit got the job done, but I definitely did not get close to 99% purity after the enrichment. I was surprised at how long and how many incubation steps there were.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Gets the job done if you are sorting your samples further.

The Bad

Long incubation steps.

The Bottom Line

If you are trying to achieve highly pure samples, I would advise against this kit. But if you are going to sort your samples further, this kit gets the job done. Just be prepared for the long and numerous incubation steps.

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