Reliable Transfection Kit

Institute of Tropical Medicine Antwerp
Virology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Thermo Fischer Scientific

Product Name:

Reliable transfection kit

Catalog Number:

L3000001

Reliable transfection kit that can be used with different cell lines. The kit contains 1 vial of Lipofectamine Reagent and 1 vial of P3000TM Enhancer Reagent. In our research, we use it mostly to transfect plasmid DNA/RNA into A549 cells to study the genomic plasticity of the Chikungunya Virus.

Experimental Design and Results Summary

Application

Transfection

Starting Material

N/A

Tips

The smallest kit is quite enough to do multiple transfections.

Results Summary

The transfection itself offers good efficiency and cells good viability. We use a GFP expressing plasmid which allows to visualize the transfected cells under the microscope (equipped for fluorescence).

DOI or PMID #

N/A

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Summary

The Good

Affordable, good efficiency and cell viability.

The Bad

None

The Bottom Line

The kit has concentrated reagents which means it is only necessary to use a small amount and the kit can be used for several transfections.

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