Western blotting via anti-Bcl-x

November 10, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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Identification of Bcl-xL protein expession using purified rabbit BCL-x antibody via Western blot.

Company:

BD Biosciences

Product Name:

Purified Rabbit Anti-Bcl-x

Catalog Number:

610212

Browse Similar Products: Anti-Bcl-x Antibodies

Anti-Bcl-x protein expression was characterized via western blot by using purified rabbit Anti-Bcl-x (1:500).

Experimental Design and Results Summary

Applications

Western blot

Sample

Lysate of murine HL-1 cells

Primary Incubation

1:500; overnight , 4°C , in TBS-T; 1%BSA; 0,02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1 h, room temperature, goat-anti-rabbit IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents

Results Summary

Using the above mentioned antibody, several bands were detected including a Bcl-xL band at approximately 30 kDa.

Additional Notes

NA

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Identification of Bcl-xL protein expession using purified rabbit BCL-x antibody via Western blot.

Summary

The Good

Bcl-xL was detected by this antibody.

The Bad

Several other bands were detected by this antibody.

The Bottom Line

This antibody is okay for protein expression analyses via Western blotting.

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