Akt/PKB protein expression analysis via Western blotting

November 13, 2014

Institut für Klinische Pharmakologie und Tox
Charité - Universitätsmedizin Berlin
PhD

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Company:

Cell Signaling Technology

Product Name:

Akt Mouse mAb

Catalog Number:

2966S

Info: View Product View Product Specs

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Akt/PKB protein expression was analyzed via Western blotting with Akt Mouse mAb (1:1000) in murine cardiomyocytes.

Experimental Design and Results Summary

Applications

Western blot

Sample

Cell lysate of murine cardiomyocytes

Primary Incubation

1:1000; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents (Amersham)

Results Summary

Akt/PKB was detected by this antibody in one distinct band (at 60 kDa)

Additional Notes

None

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Summary

The Good

One distinct band for Akt (60 kDa).

The Bad

Pricing

The Bottom Line

This antibody worked well in Western blotting.

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