Good early stem cell marker

Cardiovascular Innovation Institute
University of Louisville
Research Coordinator

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Company:

EMD Millipore

Product Name:

Monoclonal Mouse anti-Nestin

Catalog Number:

MAB353

These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the University of Louisville.  We were characterizing cells from our then-novel technique of isolating mouse oligodendrocyte precursor cells from the cortex of post-natal mice.  Since this line of cells is a progenitor line, we used quite a few different antibodies to convince ourselves that we had the cells we were looking for, at the right developmental stage.  Some of the antibodies were used to show that the cells were expressing appropriate markers; others were used to insure that we did not have inappropriate markers in our cell line, and thus in impure population.  Once we were confident in the cell line, we then co-cultured them with rat dorsal root ganglion neurons as an in vitro model of myelination.  This antibody works well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Late passage mouse oligodendrocyte precursor cells.

Primary Incubation

1:200 overnight at 4C.

Blocking Agent

PBS + 0.5% BSA + 10% NDS + 0.1% Triton

Secondary Incubation

Donkey anti-Mouse FITC at 1:100 for 1 hour at room temperature.

Tertiary Incubation

N/A

Detection

Widefield fluorescence microscopy

Results Summary

After several passages, mouse oligodendrocyte precursor cells lose their O4 specificity and "de-differentiate" and begin to express earlier stem cell markers such as nestin. This antibody worked well to show these nestin+ cells.

Additional Notes

N/A

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Summary

The Good

Specific

The Bad

NA

The Bottom Line

Nice antibody

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