New England BioLabs
Quick-Load Purple 2-Log DNA Ladder
N0550G
We run a lot of 1-2% agarose gels to test the size of our sample DNA, especially PCR products. We have used several types of ladders in the past, but this one gives an amazing range (in size) and very good quality bands on the 1% gel. Initially, it was sent to us as a sample, but we were very impressed with the results; we will switch to it from now on.
DNA size determination
1% agarose gel
The ladder is easy to use. It has a nice purple color which makes it easy to see running on the gel. The recommended volume is 10uL; however, we use 5uL and still get nice, sharp, bright bands (see photo).
Use 5uL per lane to save up!
Nice, discreet bands can be seen across a wide range (0.1-10Kb). In the image, we show the following bands (from top to bottom for the ladder on the left): 10, 8, 6, 5, 4, 3, 2, 1.5, 1.2, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2 and 0.1 kilobases). The smaller ladder on the right is from another company (for comparison). PCR products shown are 86 and 121 bp (from left to right).
None
Easy to use, bright bands, wide range
Excellent product which can be used for a large range of DNA.