EMD Millipore
Polyclonal chicken anti-GFP
AB16901
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Embryonic rat dorsal root ganglion neurons were infected with a GFP-tagged lentivirus in culture. The signal from the virus was present, but weak. Attempts to improve GFP signal with this antibody failed.
Immunofluorescence
Embryonic rat DRG neurons in culture
1:500 overnight at 4C
PBS + 0.5% BSA + 10% NDS + 0.1% Triton
Donkey anti-Chicken FITC at 1:100 for 1 hour at room temperature, protected from light
N/A
Widefield fluorescence microscopy
NA
Somewhat specific
Very faint, failed to improve GFP signal
Would not recommend