Cell Signaling
Rabbit polyclonal anti-cleaved Caspase-3
9661S
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Programmed cell death (PCD) is a critical process that underlies normal tissue development and serves as a homeostatic mechanism to maintain cell populations. The main form of PCD is known as apoptosis, which results in the activation of numerous proteolytic enzymes termed caspases. This review focuses on the detection of caspase-3, an executioner caspase for the induction of apoptosis,
Western Blot
Mouse embryonic fibroblasts (MEFs)
5% skim milk in TBST with anti-cleaved caspase-3 1/1000- 1 hour at room temperature
5% skim milk in TBST - 10 minutes at room temperature.
1/10000, room temperature for 1 hour in TBST 5% skim milk
NA
HRP- chemiluminescence
MEFS were treated with apoptotic stimuli as shown (ST, Staurosporine or E, Etoposide) and samples were subjected to Western blot analysis. Apoptosis was identified by the detection of cleaved caspase-3.
Highly specific antibody for the detection of apoptotic events!
None
Great antibody for cell death studies!