Abcam
SC35 antibody
ab28428
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SC35 protein expression was characterized via Western blot using the SC35 Antibody (1:1000).
Western blot
Cell lysate of human endothelial cells
1:1000; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3
3% BSA solution
1:5000, 1 h, room temperature, goat-anti-rabbit IgG – HRP conjugated
5% low-fat milk solution
ECL Plus Detection Reagents from Amersham
Using the above mentioned antibody (1:1000) a band was detected for SC35 at approximately 35 kDa. The antibody worked well in Western blot.
The antibody worked well in Western blot.
A band was detected for SC35 at approximately 35 kDa.
An additional band was detected at 30 kDa