MCP-1 detection in A549 cells

November 10, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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MCP-1 protein identified using MCP-1 (5) antibody via Western blot.

Company:

Santa Cruz Biotechnology, Inc.

Product Name:

MCP-1 (5) Antibody

Catalog Number:

sc-130328

Info: View Product View Product Specs

Browse Similar Products: MCP-1 Antibodies

MCP-1 protein expression was characterized via Western blot by using purified MCP-1 (C-17) Antibody

Experimental Design and Results Summary

Applications

Western blot

Sample

Cell lysate of human A549 cells

Primary Incubation

1:500; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents from Amersham

Results Summary

Using the above mentioned antibody (1:500) one band was detected for MCP-1 at approximately 12 kDa.

Additional Notes

NA

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MCP-1 protein identified using MCP-1 (5) antibody via Western blot.

Summary

The Good

There was a distinct band at approximately 12 kDa.

The Bad

Two bands in higher ranges appeared too.

The Bottom Line

Using the above mentioned antibody (1:500), one band was detected for MCP-1 at approximately 12 kDa via Western blot.

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