Phospho-p38 - Western Blot

November 21, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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Phospho-p38 protein expression identified using phoshpo-p38 MAPK antibody via Western blot.

Company:

Cell Signaling Technology

Product Name:

Phospho-p38 MAPK (Thr180/Tyr182) Antibody

Catalog Number:

9211L

Phospho- p38 protein expression was characterized via Western blotting using the Phospho-p38 MAPK (Thr180/Tyr182) Antibody from Cell Signaling Technology (1:500).

Experimental Design and Results Summary

Applications

Western blot

Sample

Cell lysate of murine HL-1 cells

Primary Incubation

1:500; overnight , 4°C , in TBS-T; 1%BSA; 0,02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1h, room temperature, goat-anti-rabbit IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents (Amersham)

Results Summary

Phospho p38 was detected by this antibody in a clear band (38 kDa).

Additional Notes

NA

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Phospho-p38 protein expression identified using phoshpo-p38 MAPK antibody via Western blot.

Summary

The Good

Phospho p38 was detected by this antibody in a clear band (38 kDa)

The Bad

Difficult to adjust the needed concentration

The Bottom Line

This antibody worked well in Western blotting.

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