APC anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody for Neutrophil cells

February 04, 2015

Cancer Biology
Scripps research center in FL
Research Associate

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Company:

BioLegend

Product Name:

APC anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Catalog Number:

108412

Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days and stained with PE-Cy7 anti-mouse CD11b Antibody and this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Bone Marrow Cultured in GM-CSF

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

After 5 days culture, approximately 60% of cells were neutrophils (CD11b+Gr-1+)

Additional Notes

None

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Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to evaluate neutrophil differentiation

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