BioLegend
Brilliant Violet 510 anti-mouse CD86 cloneGl-1
105040
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Our experiments assess the tendencies of environmental pollutants to affect dendritic cells' protein expression. We incubated Bone-Marrow Derived DCs with pollutant and collected data via flow cytometry. CD86 is a costimulatory molecule that we expect to increase in expression after incubation with the pollutant. Biolegend's BV510 is slightly difficult to optimize, which is partially due to CD86 often showing a shoulder population.
Flow Cytometry
Bone marrow derived dendritic cells
2uL of 50ug/mL per million cells for 30 minutes at 4 celsius
FC Block
NA
FACSAria II
Brilliant Violet 510 enables more sensitive assessment of CD86 expression than other fluorophores. It is difficult to optimize & uses more antibody than the Biolegend recommends per million cells.
None
Sensitivity
More protein needed
I would recommend this product, but titrate it with your own samples before using it in a real assay. The protein amount needed will likely be different than what is recommended.