MMP9 in a mouse brain tumor model

February 02, 2015

Neuro-Oncology Research
Barrow Neurological Institute
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Novus Biologicals

Product Name:

MMP9 Antibody

Catalog Number:

NBP1-57940

Info: View Product View Product Specs

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The aim with this antibody was to assess the invasive potential of glioma cells in our mouse brain tumor model.

Experimental Design and Results Summary

Applications

Western Blot

Sample

GL261-Luc2 mouse glioma tumor lysates

Primary Incubation

1:6000 in 5% BSA+TBST, overnight, 4 degrees C

Blocking Agent

5%BSA+TBST

Secondary Incubation

1:3000 in 5%BSA+TBST

Tertiary Incubation

NA

Detection

ECl Chemiluminescent Kit (Invitrogen)

Results Summary

We were able to detect bands at the expected sizes using our mouse brain tumor lysates.

Additional Notes

None

Related Categories

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Summary

The Good

Worked well without extensive optimization.

The Bad

None

The Bottom Line

This antibody worked great for our tissue samples and I would recommend it for similar studies of glioma invasive potential.

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