CD11c antibody works on tissue sections

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NEI
Lab on Immunology
Post Doc

Company:

BioLegend

Product Name:

Alexa Fluor 488 anti-mouse CD11c

Catalog Number:

117311

The antibody stains mouse cryo sections with lymphoid tissue. The tissue used is mouse retina that was cryopreserved and cryo-embedded in optical coherence tomography (note these are frozen cryosections) and then cryosectioned at 10 microns. The tissue was then stained with the antibody overnight at 4 degrees and then imaged using a confocal microscope.  The antibody was used to stain tertiary lymphoid aggregate structures in the mouse retinas which contain different types of immune cells types including CD11c+ cells which are likely dendritic cells.

Experimental Design and Results Summary

Application

Immunohistochemistry

Starting Material

Mouse tissue

Protocol Overview

Block with 2% serum, then incubate with antibody overnight at 1:500

Tips

Use concentrated amounts

Results Summary

Antibody stains lymphoid tissue

Additional Notes

None

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Summary

The Good

It works in lymphoid tissue

The Bad

Use in concentrated amounts

The Bottom Line

The antibody does work in immunohistochemistry.

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