Cell Signaling Technology
Ubc9 (D26F2) XP ® Rabbit mAb
4786S
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Protein SUMOylation is an important post-translational modification required for normal physical functions of a cell. Ubc9 is an evolutionarily conserved SUMO E2 enzyme involving in the SUMO conjugation to its substrate proteins to regulate cell cycle progression, p53 signaling pathway, and DNA damage response.
Western Blot
HeLa human cancer cell line
Dilution 1:1000 in 5% non-fat milk/TBS-Tween20. Two to four-hour incubation at room temperature.
5% non-fat milk/TBS-Tween20
HRP-conjugated anti-rabbit IgG
NA
HyBlot ES autoradiography film
HeLa cells were transfected with control and different Ubc9 siRNAs for 66 hours followed by the direct lysis of samples in 2x sample buffer. The Western blot result showed that Ubc9 was detected in control siRNA treated, but not the Ubc9 siRNA treated samples. Tubulin was used as loading control.
None
Sensitive and specific.
This is a very good antibody for detecting endogenous Ubc9.