Easy Gel Extraction Kit

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Performance

Ease-of-Optimization

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Base Pair
MOL BIO
Sr Research Assistant

Company:

Sigma Aldrich

Product Name:

Gen Elute Gel Extraction kit

Catalog Number:

NA1111-KT

Due to various factors, most PCR has primer dimers or misprimed product which needs to be removed for further downstream applications.  Our goal was to obtain one single PCR Product in a regular PCR.  This Gen Elute Gel Extraction Kit was used to purify unwanted secondary bands/primer dimers from PCR through gel extraction.  We selected this kit based on our trust in the brand name, reliability, and easy-to-follow protocall.  This kit works as expected and is a great product to perform gel extraction - yields are in the greater range and the purity of the DNA is also good .

Experimental Design and Results Summary

Tips

I do the wash step twice by halving the volume of wash buffer.

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Summary

The Good

Very good quality purification.

The Bad

Needs isopropanol which is not supplied in the kit

The Bottom Line

Quality product with quick and easy gel extraction protocol

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