PE anti-mouse F4/80 Antibody for macrophage staining

February 06, 2015

Cancer Biology
Scripps research center in FL
Research Associate

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse F4/80 Antibody

Catalog Number:

123110

Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days, then stained with anti-CD11b, Gr-1 and this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Bone Marrow Cultured in GM-CSF

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

After 5 days culture, around 30% of cells are macrophage (CD11b+Gr-1-F4/80+).

Additional Notes

None

Related Categories

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Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to evaluate macrophage differentiation.

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