Life Technologies (Invitrogen)
Rabbit anti-Connexin45
40-7000
Browse Similar Products: Connexin 45 Western Blot Antibodies
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known subpopulations - the antibodies used target known histological markers for these subpopulations. Here, I wanted to quantify connexin45 protein in brain tissue homogenates via immunoblot. This antibody showed a doublet at the appropriate weight and had more background noise than other antibodies, though most anti-connexin antibodies do. It worked for my research.
Western Blot
Brain tissue homogenate
1:250 overnight at 4 degrees C
Odyssey Blocking Buffer+Tween-20
1:10,000 for 90 minutes at room temperature
No tertiary incubation
Near-infrared fluorescence
Showed a doublet at the correct weight and had some background noise. Effective as far as connexin antibodies go.
None
Worked for the experiment and was easy to optimize.
Background may be unacceptable for some experiments.
This can work for immunoblot experiments if you require it; recommended for immunohistochemistry.