Chloride Channel Protein 2 (CLCN2) for confocal microscopy from Alomone labs

January 26, 2015

Pediatric
Johns Hopkins University
Laboratory Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Alomone Lab

Product Name:

Anti-CLC-2

Catalog Number:

ACL-002

Antibody against CLCN2 was used to investigate activation of chloride channel protein. Epithelial directed, doxycycline-regulated hCLCN2 overexpression mice were used. Few CLCN2 antibodies were commercially available. Among those tested during investigation, one (ACL-002) reviewed here produced best result for confocal microscopic analysis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Formalin-fixed, paraffin-embedded mouse nasal tissue section

Primary Incubation

1:50 dilution overnight at 4 degrees in blocking reagent, humid environment

Blocking Agent

3% BSA (fraction V, Sigma) in PBS

Secondary Incubation

1:400 dilution of anti-Rabbit Alexa Fluor 568 (Molecular Probes) in PBS for one hour at room temperature

Tertiary Incubation

NA

Detection

Zeiss LSM 510 confocal microscope

Results Summary

Antibody works fine for detecting CLCN2.

Additional Notes

None

Related Categories

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Summary

The Good

Specificity for detection

The Bad

High concentration of primary antibody (1:50) is required.

The Bottom Line

Good antibody for detecting CLCN2 in confocal microscopy analysis. Less background but it was not easy to find optimal conditions, including primary antibody dilution and laser power.

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