Swant
Goat anti-parvalbumin
PVG-214
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In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known subpopulations-the antibodies used target known histological markers for these subpopulations. I used this antibody with fluorescent secondary antibodies for immunofluorescence in fixed brain tissue. It worked well and gave great pictures.
Immunohistochemistry
PFA-fixed brain tissue slice
1:2000 for 48 hours at 4 degrees C
1% normal donkey serum in PBS (0.1% Triton-X 100)
1:1000 Cy3-conjugated donkey anti-goat for 2 hours at room temperature
NA
Cy3 fluorescence via fluorescent confocal microscopy
Staining was high-quality and easy to optimize. Staining filled cell (shown in red), including processes.
Company tests antibody against knockout tissue, increasing trustworthiness.
Detects antigen of interest, easy to optimize.
Can be expensive for the amount you get.
High-quality, expensive antibody that works well and does what it needs to do. Worth the cost to have a trustworthy antibody.