Staining of Mouse Intestinal Tissue

To analyze the intestinal tumor cells, Min+ mice were sacrificed and tumor polyps from intestinal tissue were collected and snap frozen. Cryo sections were prepared and used for staining the intestinal tumor cells. Sections were fixed and then blocked in 5% FBS buffer. Then, sections were stained with anti-β Catenin 1 antibody followed by Alexa Fluor® 488 anti-mouse IgG staining. Slides were coverslipped with mounting medium and analyzed under fluorescence microscopy. Results showed that β Catenin positive epithelia cells could be easily found with green signal in tissue samples.