The Good
CD3 staining was bright allowing easy identification of CD3-positive lymphocytes. Extracellular epitope, thus fixing and permeabilization of cell is not required. Excellent pricing. Directly conjugated with a fluorophore resulting in simple cell staining protocol. Suitable for use with additional techniques (reported by the manufacturer).
The Bad
None I can think of
The Bottom Line
This is a very good antibody to detect surface expression of CD3 on human PBMC. Bright staining of positive cells allowed easy quick and easy discrimination between positive and negative cells. Furthermore, the fact that this antibody is directly conjugated to FITC negates the use of a secondary antibody which reduces the time required for cell staining. Highly recommended.