T-bet Antibody Gives Good Staining

May 20, 2016

Immunology
University of Rochester
Graduate Student

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Quality of Results

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Company:

BioLegend

Product Name:

Alexa Fluor647 anti-T-bet antibody

Catalog Number:

644804

The aim is to determine how T-bet expression changes in T cells after different stimulations. This product was selected because BioLegend is a company that I trust and antibodies for transcription factors are only available in a few fluorochromes among different companies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T cells

Primary Incubation

Followed manufacturer's dilution. Stained in eBioscience permeabilization buffer.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

This antibody is bright enough to gate on the positive population compared to the negative control.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright

The Bad

Expensive

The Bottom Line

This antibody is able to stain T-bet and this expression is bright enough to gate.

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