A Reliable Antibody for Claudin-2 Protein Check

pathology
upmc
research associate

Overall

Quality of Results

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Company:

Life Technology

Product Name:

Claudin- 2

Catalog Number:

325600

This is a mono-antibody, the dilution used was usually from 1:500, and dilution was increased based on the background. This is a good antibody, but there is batch variance.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tissue lysis or cell line lysis

Primary Incubation

1:500 dilution, incubation overnight at 4 degrees

Blocking Agent

5% BSA or 5% non-fat milk

Secondary Incubation

1:5000-1:10000 dilution , 1hour at room temperature

Tertiary Incubation

NA

Detection

ECL

Results Summary

It's a good antibody, but there is batch variance.

Additional Notes

None

Related Categories

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Summary

The Good

A good antibody

The Bad

None

The Bottom Line

None

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