Staining of Mouse Colorectal Tumor Tissue

Microbiology and Immunology
Loyola University Chicago
Research Associate

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Company:

BioLegend

Product Name:

Purified anti-β Catenin 1 (CTNNB1) Antibody

Catalog Number:

844602

This antibody was used to analyze the expression of β Catenin in intestinal tumor cells. Intestinal tumor tissue was collected from Min+ mice and snap frozed and cryo sections were used for staining the intestinal tumor epithelial cells. Results showed that β Catenin positive epithelia cells could be easily found with strong signal in intestine tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse intestine tissue

Primary Incubation

Anti-β Catenin 1 (CTNNB1) Antibody 1: 200 incubated 4 degrees overnight

Blocking Agent

5% FBS in PBS buffer

Secondary Incubation

Alexa Fluor® 488 anti-mouse IgG 1: 250 incubate at RT 1 hour

Tertiary Incubation

DYPI containing mounting medium

Detection

Fluorescence microscopy

Results Summary

β Catenin positive epithelia cells could be easily found with strong signal in intestine tissue. The flourcense signal is also strong and specific.

Additional Notes

None

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Summary

The Good

This antibody is very specific to stain target cells.

The Bad

You may need to incubate overnight to get a strong signal

The Bottom Line

This antibody is good for IFA and can generate strong specific staining

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