R&D Systems
Human Brachyury Antibody
MAB20851
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Browse Similar Products: Brachyury Immunofluorescence Antibodies
The aim of this experiment was to detect mesodermal cells derived from human iPS cells.
Immunofluorescence
Human mesodermal cells
1/200 dilution, incubation overnight at 4 degrees
5% goat serum for 1 hour at room temperature
1/400 dilution anti-mouse alexa 488, incubation for 30 min at room temperature
None
Fluorescent microscopy
This antibody gave a strong specific nuclear signal in mesodermal cells.
Very good specificity and strong signal
I definitely recommend this antibody for immunofluorescence detection of brachyury in human mesodermal cells.