Time Course Effect of Chemoattractant on Pak2 Phosphorylation

May 19, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

Pak2

Catalog Number:

2608S

Pak2 is a family of serine/threonine protease kinase which acts as a targets for small GTP binding proteins and have been implicated in various biological activities. Here we checked the time course effect of chemoattractant on Pak2 phoshorylation.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5% BSA prepared in 1x TBST

Blocking Agent

5%BSA prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found that time dependent increase of Pak2 phosphorylation by chemoattractant.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

This antibody is very good for immunoprecipitation and Western blot.

The Bad

None

The Bottom Line

Pak2 is a important regulator for that link to Rho GTPases to cytoskeleton reorganization and nuclear signaling. This antibody is very good for immunoprecipitation and to check the serine/thronine phosphorylation of Pak2.

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