Thermo p21 Antibody (701151) Never Works

Molecular Genetics
UT Southwestern Medical Center
Research scientist

Overall

Quality of Results

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Company:

Invitrogen Antibodies

Product Name:

p21/Cip1 antibody

Catalog Number:

701151

I tested p21 expression level in Huh7 cells. After treating doxorubicin, p21 is up-regulated, which is confirmed by RT-qPCR. Compared to the p21 antibody of Santa Cruz product, Invitrogen (701151) never gives a correct size of band. p21 expression was hard to detect even with well-published p21 antibody of Santa Cruz - I had to expose it quite a long time over 30 min to detect the signal. But it worked. Anyways, I was very disappointed with the quality of p21 rabbit mAb sold by Thermo. The product webpages shows a very nice 21kDa band of cell immunoblot results. I don't understand how they got the nice images.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Huh7 cells

Primary Incubation

p21 (701151) 1:1000 overnight in cold

Blocking Agent

5% skim milk in PBS-T

Secondary Incubation

Jackson Donkey anti-rabbit IgG-HRP conjugated

Tertiary Incubation

NA

Detection

SuperECL

Results Summary

Not working at all.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Never works

The Bad

Never works

The Bottom Line

Not reliable

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