Invitrogen Antibodies
p21/Cip1 antibody
701151
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I tested p21 expression level in Huh7 cells. After treating doxorubicin, p21 is up-regulated, which is confirmed by RT-qPCR. Compared to the p21 antibody of Santa Cruz product, Invitrogen (701151) never gives a correct size of band. p21 expression was hard to detect even with well-published p21 antibody of Santa Cruz - I had to expose it quite a long time over 30 min to detect the signal. But it worked. Anyways, I was very disappointed with the quality of p21 rabbit mAb sold by Thermo. The product webpages shows a very nice 21kDa band of cell immunoblot results. I don't understand how they got the nice images.
Western Blot
Huh7 cells
p21 (701151) 1:1000 overnight in cold
5% skim milk in PBS-T
Jackson Donkey anti-rabbit IgG-HRP conjugated
NA
SuperECL
Not working at all.
None
Never works
Not reliable