BioLegend
FITC anti-mouse CD69 Antibody
104505
Info: View Product View Product Specs
Browse Similar Products: CD69 Flow Cytometry Antibodies
Good antibody anti-mouse CD69. The experiments were performed using mouse splenocytes stimulated in vitro for 3 days with 0.5ng of OKT3. Standard protocol for staining.1) Wash cells with FACS washing buffer 3x – centrifuge 5min at 300xg2) Re-suspend the pellet in 10 ul of anti-mouse CD69 diluted 1:1003) Wash 3x - centrifuge 5min at 300xg
Flow Cytometry
Mouse splenocytes
30min
NA
FACSCalibur (Becton Dickinson)
The ab titration was performed using pure Ab, 1:10, 1:30 and 1:100. After that, all the experiments were done using 1:100 dilution in 10ul.
None
Easy to use, good price
Highly recommended