Cell Signaling Technology
P-Akt 473
4060S
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This is a rabbit host antibody, and it is specific for detecting endogenous levels of Akt1 only when phosphorylated at Ser473. It also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.
Western Blot
Tissue lysis or cell lines lysis
1:1000 dilution, incubation overnight at 4 degrees
5% BSA
1:5000-1:10000 dilution, 1hour at room temperature
NA
ECL
One band with very few unspecific bands when the secondary antibody is incubated for a longer time or washing with TBST is not enough.
None
Good antibody
No specific mention, and it also could be incubated with 1% non-fat milk