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Date Published
DNA Extraction From Biosolids
(1)
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October 03, 2016
I am working on the development of molecular techniques for the detection of bacteria in the environmental samples. The PowerFecal® DNA ...
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PDCA1+ Cells Are Stained Well With This Antibody
(1)
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October 03, 2016
We wanted to study pDCs in the intestine in the normal vs tumor setting, so we used PDCA1 as one of the markers to examine frequency of pDCs
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Very Good SOCS1 Antibody
(1)
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Cell Signaling Technology
October 03, 2016
This is a very good SOCS1 antibody. Just follow the instructions of the manufacturer.. I am interested in different pathways after ...
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Use of Myosin Heavy chain (fast, perinatal) antibody from Sigma-Aldrich on myogenic cell culture
(1)
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October 03, 2016
This antibody was used to analyze the presence of myotubes in differentiated culture of mouse embryonic stem cells (mESCs). This monoclonal ...
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Antifade Mounting Medium With Nuclear Stain
(1)
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October 03, 2016
Mounting can be used to mount the fluorescently labeled sections on the glass slide. It protects the fluorescence for long time and prevents...
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Decent CD8 Antibody
(1)
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BioLegend
September 30, 2016
This is a decent CD8 antibody. Easy to use and works well at 1:50. Not as bright as I would expect from a BV650 color. Signal is maintained ...
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Perfect Tubulin Antibody
(1)
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Santa Cruz Biotechnology, Inc.
September 30, 2016
This is a very good and easy to use Tubulin Antibody. We commonly use it as a loading control if we can not use GAPDH. Gives perfect results...
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GST Purification Kit
(1)
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Bio-Rad
September 30, 2016
Rab GTPases are small GTPases of the Ras superfamily that regulate multiple processes in the secretory pathway. They cycle between GTP bound...
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The Antibody Stains CD19+ Cells In The Small Intestine Very Well
(1)
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September 30, 2016
We wanted to examine whether B cells change in the normal vs tumor setting, so we used CD19 as a marker to look at frequencies of B-cells in...
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F4/80 IF Staining In Mouse Skin
(1)
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Bio-Rad
September 30, 2016
The aim is to detect macrophages in mouse skin. The antibody is specific and easy to use.
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Staining phosphorylated STAT3 (Tyr705)
(1)
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BioLegend
September 30, 2016
In order to detect the response of cells to certain stimuli, the detection of total STAT3 is not enough. Detection of tyrosine ...
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Anti-human CD4 Antibody Review
(1)
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Tonbo Biosciences
September 30, 2016
Our lab uses a humanized mouse model generated by the intrasplenic injection of human PBMC with HIV-1 into NSG mice to test the in vivo ...
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A Nice Antibody For Gp91phox Subunit Of NOX System
(1)
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BosterBio
September 29, 2016
We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in...
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STAT3 Staining Made Easy
(1)
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BioLegend
September 29, 2016
Intracellular staining can be a bit daunting... not with this antibody! We wanted to stain human keratinocytes with STAT3 and this antibody ...
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Direct-zol RNA kit
(1)
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Zymo Research
September 29, 2016
The Direct-zol kit merges a miniprep format with Trizol RNA isolation. The benefit of the kit is that is significantly reduces the time ...
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Good Inexpensive CD8 Antibody
(1)
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Tonbo Biosciences
September 29, 2016
Antibody works great and isn't expensive. Use at 1:50. Some issues compensating this color with APC but not a huge problem.
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Good HLA-E Antibody
(1)
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Abcam
September 29, 2016
This is a good HLA-E antibody. It gives you two bands and the lower one should be the specific one. I used it to determine NK cell ...
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PCR Plate Centrifuge
(1)
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September 29, 2016
The use of this centrifuge to spin down 96 well PCR plate. Sealed plates are loaded horizontally through the upper lid. Upon closing the lid...
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EGFR antibody for Western Blotting (Cell Signaling Technology #2232)
(1)
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Cell Signaling Technology
September 29, 2016
The long-term goal of this project is to test the effect of EGFR inhibition on the proliferative properties of human primary keratinocytes. ...
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ALDH1A1 Antibody For IF Staining From Proteintech Group Inc
(1)
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Proteintech Group Inc
September 28, 2016
The aim of this study is to study the expression of enzymes regulating the retinoic acid pathway in a mouse model of colitis-associated ...
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NTF97 antibody for Western Blotting
(1)
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Abcam
September 28, 2016
The hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this ...
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Good Annexin 2 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
September 28, 2016
This is a good Annexin 2 (ANXA2) antibody. I found it to be present in my extracellular vesicles and investigated its role in membrane ...
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Alkylation kit
(1)
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September 28, 2016
Disulfide bond formation is particularly problematic for basic proteins because of the increased rate of their formation in an alkaline ...
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Good Antibody To Stain For CD161 Human Leukocytes (PE)
(1)
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BioLegend
September 28, 2016
The aim of this project was to identify CD161+ cells in human PBMCS. The marker was used to identify Th1 and Th17 cells based on surface ...
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Great CD3 Antibody
(1)
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BioLegend
September 28, 2016
I use this antibody for identification of T cells. This antibody can be used for a variety of experiments, for example identifying the ...
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CD3 Expression Of Human PBMCs
(1)
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BioLegend
September 28, 2016
This antibody was used in a flow cytometry panel in order to stain for T cells primary human PBMCs prior to identify T cell subtypes such ...
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Very Good TSG101 Antibody
(1)
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Abcam
September 27, 2016
This is a very good TSG101 antibody. I am using to determine vesicle pathway inside tumor cells. It is a very good marker for exosome as ...
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CD4 Antibody That Works, Nothing Special Though
(1)
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BioLegend
September 27, 2016
This antibody gives clear separation but is not as bright as I would expect from a BV711 color. Easy to use, works well at 1:50
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Great Heavy Duty Centrifuge With Small Footprint
(1)
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Eppendorf
September 27, 2016
The focus of our lab is the development of novel imaging agents. We use centrifuges for various applications ranging from protein extraction...
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a-Tubulin Antibody for Western Blotting
(1)
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Cell Signaling Technology
September 27, 2016
Rab proteins constitute the largest family of monomeric small GTPases that oscillate between active (GTP- bound) and inactive (GDP- bound) ...
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Anti-alpha Actin (1A4) [Immunofluorescence]
(1)
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Santa Cruz Biotechnology, Inc.
September 27, 2016
Anti-alpha Actin was to stain and detect smooth muscle cells harvested and grown from primary aortic tissue isolated from mice. Anti-alpha ...
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Good Antibody To Stain For CD161+ Human Cells
(1)
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BioLegend
September 27, 2016
The aim of our project was to identify Th1 and Th17 cells based on surface markers. Therefore we needed reliable antibodies. We chose the ...
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Review of BD anti-RIP1 (RIPK1) antibody
(1)
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BD Biosciences
September 27, 2016
Used this antibody for a western blot to detect RIP1 (RIPK1) protein levels in mouse lung. The mice whole lung were harvested, homogenized,...
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DC-SIGN Antibody For IF Staining
(1)
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DENDRITICS
September 26, 2016
The aim of this project is to study the role of immune cells in colon cancer. In the present experiment, DC-SIGN antibody was used to detect...
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Good HSV Thymidine Kinase Ab
(1)
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Santa Cruz Biotechnology, Inc.
September 26, 2016
This is a good HSV Thymidine Kinase antibody. It gives you 2 bands, where the lower one is the specific HSV Thymidine kinase, whereas the ...
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Excellent and Fast Mycoplasma PCR Detection Kit
(1)
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September 26, 2016
I used this kit to determine the relative level of contamination in my cell line after sending for a service that confirmed the presence of ...
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Good Antibody To Identify CD26+ Human PBMCs
(1)
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BD Biosciences
September 26, 2016
This antibody was used for an immune phenotypic analysis of human PBMCs in children. Due to the limited amount of blood that was available ...
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One Of The Few Human CCR7 Antibodies That Works Well
(1)
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BioLegend
September 26, 2016
I have tried many CCR7 antibodies and this is one of the few that works. Separation is ok, but when plotted against CD45RA easy to separate ...
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Western Blot Using Anti-HMOX1 Antibody (PB9212)
(1)
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BosterBio
September 26, 2016
Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted ...
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BCA Kit From Thermo Fisher For Protein Quantification
(1)
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Thermo Fisher Scientific
September 26, 2016
I work on understanding the molecular basis of hedgehog signal transduction at primary cilia. For this purpose, I manipulate the pathway at ...
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GAPDH Antibody For Western Blotting From Abcam
(1)
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Abcam
September 23, 2016
The aim of this project is to study protein-protein interactions between components of the NF-kB pathway. In this experiment, specific ...
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Eppendorf Research® plus multi-channel pipette, 0.5–10 µl, Medium Gray
(1)
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Eppendorf
September 23, 2016
We use these pipettes to set up PCR reactions in plates. They are easy to use and ergonomic.
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Good Antibody To Identify CD69+ Cells In Human Bone Marrow
(1)
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BioLegend
September 23, 2016
The aim of this study was to analyze leukocyte subsets in human bone marrow. We used this antibody because of previous good experience using...
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His-tag Protein Purification
(1)
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Bio-Rad
September 23, 2016
Rab proteins constitute the largest family of monomeric small GTPases that oscillate between active (GTP- bound) and inactive (GDP- bound) ...
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Great Small Homogenizer For Protein Extraction
(1)
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VWR Scientific Products
September 23, 2016
The focus of our research is to evaluate novel imaging agents, but we first need to pick a biomarker and determine its expression level in ...
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Detects Multiple Variants of EGFR
(1)
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Santa Cruz Biotechnology, Inc.
September 23, 2016
This is a very good total EGFR antibody. It detects all different forms of EGFR, for me especially it is important that it detects wt and ...
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Decent CD19 Antibody
(1)
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BioLegend
September 23, 2016
Separation is reasonable, not excellent, but i use this for excluding B cells from my analyses of T cells. I use it at 1:100.
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Anti-TrkA-FITC (Immunofluorescence)
(1)
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MilliporeSigma
September 22, 2016
This antibody was used to detect primary dorsal root ganglion (DRG) neurons isolated from mice. TrkA was used as a marker to detect and ...
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Good PLAU Antibody
(1)
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Santa Cruz Biotechnology, Inc.
September 22, 2016
This is an OK PLAU antibody. It gives you two bands probably for the cleaved chains of uPA. I am interested in different proteins that are ...
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Good CD45RA Antibody From Biolegend
(1)
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BioLegend
September 22, 2016
This is a reliably good anti CD45RA antibody from biolegend. BV605 is quite bright and may not be necessary for such an abundant marker. I ...
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