F4/80 IF Staining In Mouse Skin

September 30, 2016

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-rad

Product Name:

Rat anti mouse F4/80

Catalog Number:

MCA497RT

The aim is to detect macrophages in mouse skin. The antibody is specific and easy to use.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin wound

Primary Incubation

45min, RT

Blocking Agent

10% goat serum

Secondary Incubation

Goat anti rat Alexa fluor 594 45min, RT

Tertiary Incubation

None

Detection

Goat anti rat Alexa fluor 594

Results Summary

Compared to other clones of anti F4/80 we tested, this one works well on frozen sections using IF. Good alternative for CD68

Additional Notes

None

Image Gallery

Summary

The Good

Specific and fast

The Bad

None

The Bottom Line

Recommended

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