Anti-human CD4 Antibody Review

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

TONBO Biosciences

Product Name:

PerCP-Cyanine5.5 Anti-Human CD4 (OKT4)

Catalog Number:

65-0048-T025

Our lab uses a humanized mouse model generated by the intrasplenic injection of human PBMC with HIV-1 into NSG mice to test the in vivo efficacy of novel antibodies and immune activators against HIV. We perform FACS analysis to detect the different cell populations in the mouse spleens, and particularly stain for CD4 T cells, which are the target of HIV. This antibody is very useful as it comes in a color that fits in our current antibody panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1:100 for 30 min at 4 degrees Celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We were able to detect a clear population of CD4 T cells by staining for human CD3 and human CD4.

Additional Notes

None

Related Categories

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Summary

The Good

Works well and is cheaper than other antibodies

The Bad

None

The Bottom Line

I will use this antibody again

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