Very Good TSG101 Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-TSG101 antibody [4A10]

Catalog Number:

ab83

This is a very good TSG101 antibody. I am using it to determine vesicle pathway inside tumor cells. It is a very good marker for exosome as they are derived from MVBs and they should be TSG101 positive.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates from human glioma cell lines

Primary Incubation

5% milk, 4°C, overnight, shaker

Blocking Agent

5% milk, 1h, RT, shaker

Secondary Incubation

5% milk, 1h, RT, shaker

Tertiary Incubation

N/A

Detection

Bio RAD Imager

Results Summary

Good TSG101 antibody. No unspecific bands. No background

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use and good results

The Bad

None

The Bottom Line

Perfect TSG101 antibody

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