Biolegend
Anti-human CD45RA BV605
304133
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Browse Similar Products: CD45RA Flow Cytometry Antibodies
This is a reliably good anti CD45RA antibody from Biolegend. BV605 is quite bright and may not be necessary for such an abundant marker. I typically use at 1:100, best separation is at 1:50, but 1:200 still gives good signal. Signal is still good after fix/perm if performing this staining before intracellular staining
Flow Cytometry
Human PBMCs
30 min on ice
None
BD Fortessa
See two images, one for CD3+CD4+ cells the other is CD3+CD8+ cells. CD45RA shown vs CCR7 for distinguishing T cell subsets. Can see on images that it is very easy to distinguish naive/TEM/TCM/TEMRA cells.
Easy to use, good separation
Expensive
Good antibody