Decent CD19 Antibody

September 23, 2016

Immunology
Columbia University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

Anti-human CD19 BV421

Catalog Number:

302234

Separation is reasonable, not excellent, but i use this for excluding B cells from my analyses of T cells. I use it at 1:100.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human splenocytes

Primary Incubation

30 min on ice

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Fortessa

Results Summary

Image shows CD3 vs CD19 after gating on FSC and SSC. Very easy to distinguish T cells and B cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use and identify B cells

The Bad

None

The Bottom Line

Decent CD19 antibody

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