Zymo Research
Direct-zol™ RNA MiniPrep
R2050
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The Direct-zol kit merges a miniprep format with Trizol RNA isolation. The benefit of the kit is that is significantly reduces the time needed to purify RNA samples when lysing cells or tissues with Trizol, RNAzol, or any other TRI reagent.
RNA isolation
Tissue culture cells
I simply followed the mfr's protocol.
A cell scraper is very handy when lysing monolayers from a tissue culture plate.
As can be seen in Figure 1, RNA bands are distinct with little gDNA contamination. This experiment yielded about 200 ng/uL of RNA (about 10 ug total) from a single well of a 12 well tissue culture plate. In Figure 2, four separate RT-PCR reactions were performed using the purified RNA. The BTF3 product was the best yield; DRG1 less so [note arrow]. The other two reactions require optimization.
For RT-PCR, I strongly recommend the in-column DNAase treatment.
Reduces the amount of toxic organic solvent waste
None
Given that standard guanidine containing RNA miniprep kits have finite lives, the Direct-zol kit is perfect for a lab performing infrequent RNA isolations.
Product Name: Direct-zol™ RNA MiniPrepSupplier Page