Detects Multiple Variants of EGFR

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Santa Cruz Biotech

Product Name:

EGFR (1005)-G Antibody

Catalog Number:

sc-03-G

This is a very good total EGFR antibody. It detects all different forms of EGFR, for me especially it is important that it detects wt and vIII variant. I am interested in multiple driver mutations in cancer.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates from human glioma cell lines

Primary Incubation

5% milk, 4°C, overnight, shaker

Blocking Agent

5% milk, 1h, RT, shaker

Secondary Incubation

5% milk, 1h, RT, shaker

Tertiary Incubation

N/A

Detection

Bio Rad Imager

Results Summary

Good EGFR antibody. Detects both wt and vIII variant. I have also used it for IF and it works very well.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use. Detects multiple variants

The Bad

Different bands can be confusing

The Bottom Line

Good EGFR antibody.

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