ALDH1A1 Antibody For IF Staining From Proteintech Group Inc

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech Group Inc

Product Name:

ALDH1A1 Antibody

Catalog Number:

15910-1-AP

The aim of this study is to study the expression of enzymes regulating the retinoic acid pathway in a mouse model of colitis-associated colon cancer model. In this experiment, the expression of ALDH1A1 was tested in a normal colon tissue of mouse. This is a pilot experiment to test the antibody for IF purpose.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse colon tissue sample

Primary Incubation

1:100 dilution in blocking buffer; over night incubation at 4 deg Celsius

Blocking Agent

1x PBS + 1% BSA + 1% goat serum

Secondary Incubation

1:200 dilution (in 1xPBS + 1% BSA) of Goat-anti rabbit Alexa dye 594; 1 hour at r/t

Tertiary Incubation

No

Detection

Confocal Imaging

Results Summary

ALDH1A1 expression could be nicely detected in the mouse colon tissue section

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific signal

The Bad

No

The Bottom Line

Works well for IF staining

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